Adeno-Associated Virus (AAV) Vectors in Gene Therapy by K. I. Berns, C. Giraud (auth.), Professor Kenneth I. Berns

By K. I. Berns, C. Giraud (auth.), Professor Kenneth I. Berns M.D., Ph.D., Catherine Giraud Ph.D. (eds.)

Human gene treatment holds nice promise for the therapy of many genetic ailments. as a way to in achieving any such treatment there are necessities. First, the affected gene needs to be cloned, its se­ quence made up our minds and its rules safely characterised. moment, an appropriate vector for the supply of a great reproduction of the affected gene has to be on hand. For a vector to be of use numerous attributes are hugely fascinating: those contain skill to hold the intact gene (although this can be both the genomic or the cDNA shape) in a strong shape, skill to introduce the gene into the specified mobile sort, skill to precise the brought gene in an accurately regulated demeanour for a longer time period, and a scarcity of toxicity for the recipient. additionally of shock is the frequency of cellphone transformation and, occasionally, the facility to introduce the gene into nondividing stem cells. Sev­ eral animal viruses were demonstrated as power vectors, yet none has confirmed to have the entire wanted houses defined above. for instance, retroviruses are tricky to propagate in adequate titers, don't combine into nondividing cells, and are of outrage as a result of their oncogenic homes in a few hosts and since they combine at many websites within the genome and, hence, are possibly insertional mutagens. also, genes brought through retroviral vectors are usually expressed for really brief classes of time. A moment virus used as a vector in version platforms has been adenovirus (Ad).

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Extra resources for Adeno-Associated Virus (AAV) Vectors in Gene Therapy

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VP-2 utilizes an ACG triplet as a initiation codon upstream from the start of VP-3 1991, 1992; SAMULSKI et al. 1991). , when adenovirus superinfects the cell), the proviral rep and cap genes are expressed and viral replication proceeds. 2 Effects of Rep on Gene Regulation Regulation of AAV gene expression is complex and involves positive and negative regulation of viral transcription. The two larger nonstructural proteins of AAV, Rep68 and Rep78, are required for this regulation. These proteins have been shown to bind to ITRs (ASHKTORAB and SRIVASTAVA 1989; CHIORINI et al.

1993). DNA sequence analysis and extensive mutagenesis of AAV has revealed that between the terminal repeat sequences are two large translation open reading frames (SRIVASTAVA et al. 1983; HERMONAT et al. 1984; TRATSCHIN et al. 1984a). The reading frame on the right side of the genome contains the capsid (cap) gene which encodes the three structural proteins, VP-1, -2 and -3 (Fig. 1). These proteins make up the virion and are translated from two differentially spliced mRNAs that originate from the transcription promoter at map unit 40, p40.

The inefficient cleavage with the linear substrates may be sufficient for the cellular effects associated with Rep. A. Chiorini et al. Rep effects expression of promoters lacking a Rep binding site. This repression, not attributable to Rep promoter interactions, constitutes a nonspecific effect. Transient expression assays indicate that Rep may also function posttranscriptionally to repress gene expression, as indicated by increased levels of transcripts and a concomitant decrease of reporter gene product activity (unpublished observations; TREMPE and CARTER 1988).

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