By Jonathan Y. Richmond, Robert W. McKinney
Describes the combos of normal & distinctive microbiological practices, safeguard equip., & amenities that are prompt for paintings with various infectious brokers in quite a few labs. This revision takes under consideration such concerns as worldwide predicament approximately rising & reemerging infectious illnesses; elevated curiosity in prion illnesses with the id of BSE in England; the prevalence of numerous lab-associated infections; elevated drawback in regards to the nationwide & intl. move of infectious microorganisms; & turning out to be issues approximately bioterrorism, & the elevated protection wishes of our microbiological labs.
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Extra resources for Biosafety in Microbiological and Biomedical Laboratories
Outer and inner change rooms separated by a shower are provided for personnel ente ring a nd lea ving th e suit area . A sp ecia lly designe d suit area is main tained in the facility to provide personnel protection equivalent to that provided by Class III bio logical safe ty cabinets. Personnel who enter this area wear a one-piece positive pressure suit that is ventilated by a lifesupport system protected b y HEPA filtration. The life sup port s ystem includ es re dun dan t brea thing air compressors, alarms and emergency backup breathing air tanks.
All cultures, stocks, and other regulated wastes are decontaminated before disposal by an approved decontamination method such as autoclaving. Materials to be decontam inated outside of the imme diate laboratory are to be placed in a durable, leakproof container and closed for transport from the laboratory. Materials to be decontaminated outside of the immediate laboratory are packaged in accordance with applicable local, state, and fede ral reg ulatio ns be fore rem oval f rom the fa cility. 9.
Special Practices 1. Only persons whose presence in the facility or individual laboratory rooms is required for program or support purposes are authorized to enter. Persons who are immunocomprom ised or immunosuppressed m ay be at risk of acquiring infections. Therefore, persons who may be at increased risk of acquiring infection or for whom infection may be unusually hazardous, such as children or pregnant women, are not allowed in the laboratory or anim al room s. The supervisor has the final responsibility for assessing each circumstance and determining who may enter or work in the laboratory.