By Sukriti Nag
Univ. of Toronto, Canada. textual content offers the main points of chosen morphologic, permeability, delivery, in vitro, and molecular strategies for BBB reviews. every one half is preceded by way of a overview emphasizing the benefits and pitfalls of specific thoughts. Written for researchers in BBB. DNLM: Blood--Brain Barrier--physiology.
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Additional info for Blood'Brain Barrier: Biology and Research Protocols
Res. 56, 65–77. 264. Esiri, M. , and McGee, J. (1986) Monoclonal antibody to macrophages (EBM/11) labels macrophages and microglial cells in human brain. J. Clin. Pathol. 39, 615–621. 265. , and Dore-Duffy, P. (1996) CNS microvascular pericytes express macrophage-like function, cell surface intergrin alphaM, and macrophage marker ED-2. Microvasc. Res. 52, 127–142. 266. Graeber, M. , Streit, W. , and Kreutzberg, G. W. (1989) Identity of ED2-positive perivascular cells in rat brain. J. Neurosci. Res.
And Hart, M. N. (1988) Composition and mechanics of cerebral arterioles in hypertensive rats. Am. J. Pathol. 133, 464–471. 6. Gross, P. , Sposito, N. , Pettersen, S. , and Fenstermacher, J. D. (1986) Differences in function and structure of the capillary endothelium in gray matter, white matter and a circumventricular organ of rat brain. Blood Vessels 23, 261–270. 7. , and Kilty, D. W. (1997) Cerebrovascular changes in chronic hypertension. Protective effects of enalapril in rats. Stroke 28, 1028–1034.
Shorter periods result in poor preservation and fragile slices. If brain blocks or slices are immersion fixed for longer than 48 h there is extraction of tissue constituents and organelles and nerve processes appear swollen. The advantage of en bloc staining is that preservation of membranes is superior (see Figs. 2C and D), and it eliminates having to stain grids with uranyl acetate prior to viewing. The latter technique is more likely to produce precipitates on sections making them unsuitable for photography.