By Bernard Roizman (auth.), W. Arber, W. Braun, F. Cramer, R. Haas, W. Henle, P. H. Hofschneider, N. K. Jerne, P. Koldovsky, H. Koprowski, O. Maaløe, R. Rott, H.-G. Schweiger, M. Sela, L. Syruček, P. K. Vogt, E. Wecker (eds.)
"When we supply a definition it truly is for the aim of utilizing it". HENRI POINCARE in technology and approach A. targets the 1st model of this paper was once written to introduce new scholars and fellows of my laboratory to the mysteries of herpesviruses. Consonant with this layout sections facing good documented information have been trimmed to the bone while many vague phenomena, debatable information and probably trivial observations have been mentioned generously and at size. there's a few doubt to whether it used to be intended to be released, however it used to be now not a evaluation. the target of stories is usually to carry order. yet lamentably, even the main fluent summation of credible facts usually makes boring studying and an excessive amount of believable order, like little or no entropy in chemical reactions, isn't the most fitted atmosphere on which to nurture the urge to find. This model is extra charitable yet now not much less inbalanced. The bibliography displays the purpose of the paper and was once up-to-date final in December of 1968. it's going to be seen with no asserting that no unmarried account equivalent to this may do justice or injustice, because the case can be, to the different hundred papers released on herpesviruses every year or to the numerous thousand papers released on herpesviruses because the first of the family members used to be experimentally transmitted to a heterologous host greater than part a century in the past (GRUTER, 1924). B. Definition 1.
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Additional resources for Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitatsforschung
The ducts as seen by the electron microscope and fibrils as seen by light microscopy were absent from uninfected cells and from DK cells abortively infected with the same virus. Several comments concerning the structure and function of the tubules and the release of virus from cells seem appropriate. (i) The tubules are not seen in uninfected HEp-2 cells. Nevertheless, the source of genetic information for the tubules is unknown. FELLUGA (196)) suggested that the structures bounded by membranes and countaining enveloped nucleocapsids are a host reaction to infection.
RorZMAN: + largely to the fact that the DNAs of the two viruses have high G C molar base ratios and are readily separated from cellular DNA by isopycnic centrifugation in CsCI density gradients (Table 1). It is perhaps relevant to note that very little is known concerning the synthesis of viral DNAs more closely approximating host DNA with respect to base ratios. The most extensive studies were done on pseudorabies DNA synthesis in rabbit kidney cells (KAPLAN and BEN-PORAT, 1967, 1963, 1964, 1966a,b; KAPLAN, 1964; BEN-PORAT and KAPLAN, 1963).
A) Pattern of Synthesis of Viral DNA. Two basic methods have been used to estimate the pattern of synthesis of viral DNA. The first involves determination of specific activity of viral DNA in cells pulse-labeled with radioactive thymidine at intervals after infection. The pattern obtained for herpes simplex virus DNA (SYDISKIS and ROIZMAN, unpublished studies) synthesis in HEp-2 cells is shown in Fig. 5. The salient features are that within a few hours after infection host DNA synthesis is almost completely replaced by viral DNA synthesis.