DNA Methods in Clinical Microbiology by P. Singleton

By P. Singleton

DNA equipment in medical Microbiology describes the unconventional DNA-based expertise now utilized in the prognosis and administration of infectious ailments. it's a concise, but readable, evaluation written essentially for clinicians, medical microbiologists, scientific scholars and undergraduates in scientific and veterinary microbiology.
The ebook has fundamental goals. First, to provide an explanation for the rules of those equipment on the `molecular' point. moment, to supply a scientific viewpoint via reporting effects from genuine DNA-based investigations on a variety of specimens.
these impending DNA tools for the 1st time are assisted through a quick résumé of the relevant good points of nucleic acids (Chapter 2): this knowledge is vital for an knowing of later chapters. next textual content covers detection, characterization and quantification of pathogens by means of a number of equipment - e.g., objective amplification (PCR, LCR, NASBA, TMA and SDA), sign amplification (bDNA) and probe-based innovations; the bankruptcy on typing describes approximately twenty named molecular equipment, together with spoligotyping and MLST. All chapters contain an enough diversity of present reference from which, if required, specific protocols will be bought. The diagrams are transparent, and readers are assisted by means of an in depth index.

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Journal of Clinical Microbiology 37: 828-829. Jersek B, Gilot P, Gubina M et al (1999) Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR. Journal of Clinical Microbiology 37: 103-109. Jorgensen JH, Salinas JR. Paxson R et a! ( 1999) False-positive Gen-Probe Direct Mycobacterium tuberculosis Amplification Test r,esults from patients with pulmonary M. kansasii and M. avium infections. Journal of Clinical Microbiology 37: 175-178. Kauppinen J. rvi R & Katila M-L ( 1999) Mycobacterium malmoense-specific nested PCR based on a conserved sequence detected in random amplified polymorphic DNA fingerprints.

Schmitz F-J, MacKenzie CR, Hofmann B eta! (1997) Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR. Journal of Medical Microbiology 46: 773-778. NUCLEIC ACIDS, TARGET SEQUENCES, SPECIMENS 43 Schmitz F-J, Steiert M, Hofmann B et al ( 1998a) Development of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin-1 in Staphylococcus aureus isolates. Journal of Medical Microbiology 47: 335-340.

Appuhamy S, Parton R, Coote JG & Gibbs HA (1997) Genomic fingerprinting of Haemophilus somnus by a combination of PCR methods. Journal of Clinical Microbiology 35: 288-291. Boivin G, Handfield J, Toma L et al (1999) Expression of the late cytomegalovirus (CMV) pp 150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in plasma. Journal of Infectious Diseases 179: 1101-1107. NUCLEIC ACIDS, TARGET SEQUENCES, SPECIMENS 39 Bolton LF, Kelley LC, Lee MD et al {1999) Detection of multidrug-resistant Salmonella enterica serotype typhimurium DT104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol.

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